University of Freiburg Medical Centre

2 ARTICLES PUBLISHED IN JoVE

Medicine

Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle

Jan R. Thiele¹, Kurt Goerendt¹, G. Bjoern Stark¹, Steffen U. Eisenhardt¹

¹Department of Plastic and Hand Surgery, University of Freiburg Medical Centre

Digital intravital epifluorescence microscopy of postcapillary venules in the cremasteric microcirculation is a convenient method to gain insights into leukocyte-endothelial interaction in vivo in ischemia-reperfusion injury (IRI) of striated muscle tissue. We here provide a detailed protocol to safely perform the technique and discuss its applications and limitations.

Medicine

Single-stage Dynamic Reanimation of the Smile in Irreversible Facial Paralysis by Free Functional Muscle Transfer

Jan Thiele¹, Holger Bannasch¹, G. Bjoern Stark¹, Steffen U. Eisenhardt¹

¹Department of Plastic and Hand Surgery, University of Freiburg Medical Centre

The use of the masseteric nerve as donor nerve represents a single-stage alternative to the criterion standard two-stage procedure of cross-facial nerve grafting and free muscle transfer in facial paralysis. We provide a detailed description to safely perform this technique with a gracilis muscle transfer and discuss indications and limitations.