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Keck School of Medicine of the University of Southern California

6 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Tissue Engineering of the Intestine in a Murine Model
Erik R. Barthel 1, Allison L. Speer 1, Daniel E. Levin 1, Frédéric G. Sala 1, Xiaogang Hou 1, Yasuhiro Torashima 1, Clarence M. Wigfall 1, Tracy C. Grikscheit 1
1Children's Hospital Los Angeles, Division of Pediatric Surgery, Saban Research Institute, Keck School of Medicine of the University of Southern California

This article and the accompanying video present our protocol for generating tissue-engineered intestine in the mouse, using an organoid units-on-scaffold approach.

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Medicine

Whole Vitreous Humor Dissection for Vitreodynamic Analysis
Karthik Murali 1, Amir H. Kashani 2, Mark S. Humayun 1
1Biomedical Engineering, University of Southern California, 2Ophthalmology, University of Southern California

The goal of this protocol is to show an effective technique to isolate whole, intact vitreous core and cortex from post mortem enucleated porcine eyes.

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Developmental Biology

Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs
Ksenia Gnedeva 1,2, A. J. Hudspeth 3, Neil Segil 1,2
1Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of the University of Southern California, 2Caruso Department of Otolaryngology-Head and Neck Surgery, Keck School of Medicine of the University of Southern California, 3Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience, The Rockefeller University

Three-dimensional organotypic cultures of the murine utricle and cochlea in optically clear collagen I gels preserve innate tissue morphology, allow for mechanical stimulation through adjustment of matrix stiffness, and permit virus-mediated gene delivery.

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Neuroscience

Retinal Vascular Reactivity as Assessed by Optical Coherence Tomography Angiography
Sam Kushner-Lenhoff 1, Bright S. Ashimatey 1, Amir H. Kashani 1,2
1Department of Ophthalmology, USC Roski Eye Institute, 2USC Ginsberg Institute for Biomedical Therapeutics, Keck School of Medicine of the University of Southern California

This article describes a method for measuring retinal vasculature reactivity in vivo with human subjects using a gas breathing provocation technique to deliver vasoactive stimuli while acquiring retinal images.

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Cancer Research

Aqueous Humor as a Liquid Biopsy for Retinoblastoma: Clear Corneal Paracentesis and Genomic Analysis
Mary E. Kim 1,2, Liya Xu 1,2,3,11, Rishvanth K. Prabakar 4, Lishuang Shen 5, Chen-Ching Peng 1,3, Peter Kuhn 3,6,7,8, Xiaowu Gai 5,9, James Hicks 3,6,10, Jesse L. Berry 1,2,6,11
1The Vision Center, Children’s Hospital Los Angeles, 2USC Roski Eye Institute, Keck School of Medicine of the University of Southern California, 3Department of Biological Sciences, Dornsife College of Letters, Arts, and Sciences, University of Southern California, 4Department of Molecular and Computational Biology, University of Southern California, 5Center for Personalized Medicine, Department of Pathology and Laboratory Medicine, Children’s Hospital Los Angeles, 6Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, 7Department of Aerospace and Mechanical Engineering, Viterbi School of Engineering, University of Southern California, 8Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, 9Department of Pathology and Laboratory Medicine, Keck School of Medicine of USC, 10Department of Biochemistry and Molecular Medicine, Keck School of Medicine, University of Southern California, 11The Saban Research Institute, Children’s Hospital Los Angeles

The aqueous humor is a high-yield liquid biopsy for retinoblastoma, intraocular cancer that cannot be biopsied in vivo due to the risk of extraocular spread. Herein, a method for safely extracting aqueous humor via clear corneal paracentesis and steps for genomic analysis to identify prognostic biomarkers are presented.

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Medicine

Development of a Surgical Technique for Subretinal Implants in Rats
Juan Carlos Martinez Camarillo 1,2, Yuntao Hu 1,5, Biju B. Thomas 1,2, Danhong Zhu 3, David R. Hinton 2,3, Debbie Mitra 1,2, Jose Miguel Mora Correa 2, Deepthi S. Rajendran Nair 1,2, Jane Lebkowski 4, Mark S. Humayun 1,2
1USC Roski Eye Institute, Keck School of Medicine of the University of Southern California, 2USC Dr. Allen and Charlotte Ginsburg Institute for Biomedical Therapeutics, University of Southern California, 3Department of Pathology, Keck School of Medicine of the University of Southern California, 4Regenerative Patch Technologies LLC, 5Eye center, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University

The present protocol describes the scleral approach for subretinal device implantation, a feasible surgical technique for implementation in animal models of retinal diseases in research.

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