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Bond University

1 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation
Karin Tourle 1, Amber Rucinski 1, Alexander Grainger 1, Ioannis J. Limnios 1, Macarena Gonzalez Ruiz 1, Jonathan K.H. Tan 1
1Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine, Bond University

This article describes a protocol for aggregating and encapsulating spleen cells within a semi-solid basement membrane matrix. Basement membrane matrix constructs can be used in three-dimensional culture for studying organoid development, or for in vivo transplantation and tissue regeneration studies.

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