Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue FormationKarin Tourle 1, Amber Rucinski 1, Alexander Grainger 1, Ioannis J. Limnios 1, Macarena Gonzalez Ruiz 1, Jonathan K.H. Tan 1
1Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine, Bond University
This article describes a protocol for aggregating and encapsulating spleen cells within a semi-solid basement membrane matrix. Basement membrane matrix constructs can be used in three-dimensional culture for studying organoid development, or for in vivo transplantation and tissue regeneration studies.
