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Jianghan University

4 ARTICLES PUBLISHED IN JoVE

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Medicine

Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System
Wusong Zou 1, Yanqun Yang 1, Ye Gu 1, Pengfei Zhu 1, Mingjing Zhang 1, Zhong Cheng 1, Xiaoyan Liu 1, Yijun Yu 1, Xiaohong Peng 1
1Heart Center at Puai Hospital, Wuhan Puai Hospital, Jianghan University

Here, we describe a simple tail vein blood sampling method in non-anesthetized rats using a vacuum extraction tube system. This method reduces the risk of direct exposure to blood and simplifies taking multiple samples from a single venipuncture.

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Behavior

Modified Blood Collection from Tail Veins of Non-anesthetized Mice with a Vacuum Blood Collection System and Eyeglass Magnifier
Xiaoyan Liu *1, Huanhuan Li *1, Jie Wu 1, Yijun Yu 1, Mingjing Zhang 1, Wusong Zou 1, Ye Gu 1
1Heart Center, Wuhan Fourth Hospital, Puai Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology

This study reports blood sampling from tail vein in mice using a vacuum extraction tube system with eyeglass magnifier. Our method is easy to practice and could be used for repeat blood sampling in mice.

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Neuroscience

Induction of Acute Ischemic Stroke in Mice Using the Distal Middle Artery Occlusion Technique
Changlong Leng *1, Youwei Li *1, Yaojian Sun 1, Wei Liu 1
1Wuhan Institute of Biomedical Sciences, School of Medicine, Jianghan University

Here, we present a protocol to establish a distal middle cerebral artery occlusion (dMCAO) model through transcranial electrocoagulation in C57BL/6J mice and evaluate the subsequent neurological behavior and histopathological features.

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Medicine

A Mouse Model of Hemorrhagic Transformation Induced by Acute Hyperglycemia Combined with Transient Focal Ischemia
Yaojian Sun *1, Changlong Leng *1, Kang Ma 1, Wei Liu 1
1Hubei Key Laboratory of Cognitive and Affective Disorder, Institute of Biomedical Sciences, School of Medicine, Jianghan University

This study presents a protocol for establishing a highly reproducible animal model of hemorrhagic transformation (HT) using middle cerebral artery occlusion/reperfusion (MCAO/R) in C57BL/6 mice with acute hyperglycemia.

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