Kyoto University Hospital

3 ARTICLES PUBLISHED IN JoVE

Medicine

Orthotopic Small Bowel Transplantation in Rats

Koji Kitamura^*1,2, Martin W. von Websky^*1, Ichiro Ohsawa¹, Azin Jaffari¹, Thomas C. Pech¹, Tim Vilz¹, Sven Wehner¹, Shinji Uemoto², Joerg C. Kalff¹, Nico Schaefer¹

¹Department of Surgery, University of Bonn, Germany, ²Department of Surgery, Kyoto University Hospital

Small bowel transplantation has become an accepted treatment option for patients with irreversible intestinal failure. Our experimental model of orthotopic small bowel transplantation in rats serves as a reliable tool to address underlying immunologic and inflammatory processes that complicate intestinal transplantation.

Medicine

Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction

Kazuyuki Nagai^1,2, Shintaro Yagi², Shinji Uemoto², Rene H. Tolba¹

¹Institute for Laboratory Animal Science and Experimental Surgery, RWTH-Aachen University, ²Department of Hepato-Biliary-Pancreatic Surgery and Transplantation, Graduate School of Medicine, Kyoto University

Orthotopic liver transplantation in rats is an indispensable experimental model for biomedical research. Here we present our surgical procedures for orthotopic rat liver transplantation with hepatic arterial reconstruction using a 50% partial graft.

Biology

Picrosirius Red Staining for Semiquantitative Histopathologic Evaluation of Collagen Deposition in Murine Models of Chronic Lung Allograft Rejection

Birte Ohm^*1, Julia Thomé^*2, Thierry Christmann², Maike Lind², Yoshito Yamada³, Andreas Spörlein⁴, Laura Anna Schneider², Steffen U. Eisenhardt², Wolfgang Jungraithmayr¹

¹Department of Thoracic Surgery, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, ²Department of Plastic and Hand Surgery, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, ³Department of Thoracic Surgery, Kyoto University Hospital, ⁴Department of Otolaryngology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg

Picrosirius Red staining is a semiquantitative method for objectively assessing collagen deposition in murine pulmonary fibrotic remodeling in a spatially resolved manner. Apart from evaluating total collagen deposition, Picrosirius Red staining allows for differentiating collagen fibers of different thicknesses.