Fujian Agriculture and Forestry University

6 ARTICLES PUBLISHED IN JoVE

Biochemistry

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

Bidhan Chandra Nayak^*1, Jie Wang^*1, Lianyun Lin^*1,2,3,4, Weiyi He^2,3,4, Minsheng You^2,3,4, Zhiguang Yuchi^1,2

¹Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Collaborative Innovation Center of Chemical Science and Engineering, School of Pharmaceutical Science and Technology, Tianjin University, ²State Key Laboratory of Ecological Pest Control for Fujian/Taiwan Crops and Institute of Applied Ecology, Fujian Agriculture and Forestry University, ³Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou, ⁴Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University

In this article, we describe the protocols of protein expression, purification, crystallization and structure determination of the N-terminal domain of ryanodine receptor from diamondback moth (Plutella xylostella).

Environment

Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle

Yan-Ru Zhang^1,2, Hai-Li Qiao³, Li-Li Ren¹, Rong Wang⁴, Peng-Fei Lu¹

¹The Key Laboratory for Silviculture and Conservation of the Ministry of Education, School of Forestry, Beijing Forestry University, ²School of Forestry, Inner Mongolia Agricultural University, ³Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, ⁴School of Forestry, Fujian Agriculture and Forestry University

In order to observe ultrastructure of insect sensilla, scanning and transmission electron microscopy (SEM and TEM, respectively) sample preparation protocol were presented in the study. Tween 20 was added into the fixative to avoid sample deformation in SEM. Fluorescence microscopy was helpful for improving slicing accuracy in TEM.

Medicine

Invasive Hemodynamic Assessment for the Right Ventricular System and Hypoxia-Induced Pulmonary Arterial Hypertension in Mice

Fuliang Luo¹, Xin Wang¹, Xiaokang Luo¹, Bo Li¹, Desheng Zhu², Hansong Sun³, Yue Tang¹

¹Beijing Key Laboratory of Pre-clinical Research and Evaluation for Cardiovascular Implant Materials, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Centre for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, ²Laboratory Animal Center, Peking University, ³Center of Cardiac Surgery for Adults, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Centre for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College

Here, we present a protocol to perform an invasive hemodynamic assessment of the right ventricle and pulmonary artery in mice using an open-chest surgery approach.

Medicine

Technical Modification of the Terminal Ureter During Total Transperitoneal Laparoscopic Nephroureterectomy for Upper Urinary Tract Urothelial Carcinoma

Chunlong Fu^*1,2, Yaqun Zhang^*1, Xin Wang¹, Shengcai Zhu¹, Huimin Hou¹, Shengjie Liu¹, Cheng Pang¹, Xinda Song¹, Jia Chen¹, Jianye Wang¹, Ming Liu^1,2

¹(Urology) Beijing Hospital, National Center of Gerontology, The Joint Unit of the Chinese Academy of Medical Sciences, ²Graduate School of Peking Union Medical College, Beijing Hospital

Here, we present a protocol to increase the surgical field of view and reduce the difficulty of total transperitoneal laparoscopic nephroureterectomy surgery by precutting the umbilical ligament before treating the terminal ureter.

Biology

Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host

Yanfei Wang¹, Xin Wang¹, Zhiqiang Li¹, Qian Chen¹

¹Vector-borne Virus Research Center, Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University

This protocol demonstrates how to use the plant host to detect salivary proteins of leafhopper and plant viral proteins released by leafhopper vectors.

Neuroscience

Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells

Sue Han^*1, Xin Wang^*1, Nicholas Cordero¹, Ling-Gang Wu¹

¹National Institute of Neurological Disorders and Stroke

This protocol describes a confocal imaging technique to detect three fusion modes in bovine adrenal chromaffin cells. These fusion modes include 1) close-fusion (also called kiss-and-run), involving fusion pore opening and closure, 2) stay-fusion, involving fusion pore opening and maintaining the opened pore, and 3) shrink-fusion, involving fused vesicle shrinkage.