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US Army Research Laboratory

2 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
Deborah A. Sarkes 1, Justin P. Jahnke 1, Dimitra N. Stratis-Cullum 1
1Sensors and Electron Devices Directorate, US Army Research Laboratory

Biopanning bacterial display libraries is a proven technique for discovery of peptide affinity reagents, a robust alternative to antibodies. The semi-automated sorting method herein has streamlined biopanning to decrease the occurrence of false positives. Here we illustrate the thought process and techniques applied in evaluating candidates and minimizing downstream analysis.

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Engineering

Processing of Bulk Nanocrystalline Metals at the US Army Research Laboratory
Vincent H. Hammond 1, Billy C. Hornbuckle 1, Anit K. Giri 1, Anthony J. Roberts 1, Thomas L. Luckenbaugh 2, Joseph M. Marsico 3, Scott M. Grendahl 1, Kristopher A. Darling 1
1Weapons and Materials Research Directorate, US Army Research Laboratory, 2Bowhead Total Enterprise Solutions, LLC, 3Oak Ridge Institute for Science and Education

This paper provides a brief overview of the ongoing efforts at the Army Research Laboratory on the processing of bulk nanocrystalline metals with an emphasis on the methodologies used for the production of the novel metal powders.

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