Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron MicroscopyTilman Franke 1, Irina Kolotuev 2
1Thermo Fisher, 2Universite de Lausanne
We describe the preparation of ribbons of serial sections and their collection on large transfer support for use as Array Tomography samples, along with automated imaging procedures in a scanning electron microscope. The protocol allows screening, retrieval, and targeted imaging of local, rare events, and the acquisition of large data volumes.
