University of wurzburg

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model

Malgorzata Burek¹, Ellaine Salvador¹, Carola Y. Förster¹

¹Klinik und Poliklinik für Anästhesiologie, University of Wurzburg

This method describes how to isolate and immortalize microvascular endothelial cells from mouse brain. We describe a step-by-step protocol starting from the homogenization of brain tissue, digestion steps, seeding and immortalization of the cells. Usually, it takes about five weeks to obtain a homogenous, immortalized microvascular endothelial cell line.

Medicine

Stretch in Brain Microvascular Endothelial Cells (cEND) as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier

Ellaine Salvador¹, Winfried Neuhaus^1,2, Carola Foerster¹

¹Klinik und Poliklinik für Anästhesiologie, Zentrum für operative Medizin der Universität Würzburg, ²Department of Medicinal Chemistry, Faculty of Life Sciences, University of Vienna

In vitro traumatic brain injury models are being developed to reproduce in vivo brain deformation. Stretch-induced injury has been employed for astrocytes, neurons, glial cells, aortic, and brain endothelial cells. However, our system uses a blood brain barrier (BBB) model that possesses properties constituting a legitimate model of the BBB to establish an in vitro TBI model.