Efficient Genome Editing of Mice by CRISPR Electroporation of ZygotesChantal K. Diallo 1, Andrew J. Modzelewski 1
1Department of Biomedical Sciences, School of Veterinary Medicine, The University of Pennsylvania
Here, we describe a simple technique intended for the efficient generation of genetically modified mice called CRISPR RNP Electroporation of Zygotes (CRISPR-EZ). This method delivers editing reagents by electroporation into embryos at an efficiency approaching 100%. This protocol is effective for point mutations, small genomic insertions, and deletions in mammalian embryos.
