A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein PreservationJon Waters 1, Vishal Dhere 1, Adam Benjamin 1, Arvind Sekar 1, Archana Kumar 1, Sampath Prahalad 2, David T. Okou 1, Subra Kugathasan 1
1Division of Gastroenterology, Hepatology and Nutrition, Department of Pediatrics, Emory University School of Medicine and Children's Health Care of Atlanta, 2Division of Rheumatology, Department of Pediatrics, Emory University School of Medicine and Children's Health Care of Atlanta
We are describing a new method of isolating genomic DNA from whole blood collected for plasma/serology. After plasma collection, the compacted blood is usually discarded. Our novel method represents a significant improvement over existing methods and makes DNA and plasma available from a single collection, without requesting additional blood.