Southwest University

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

Stimulation Location Determination using a 3D Digitizer with High-Definition Transcranial Direct Current Stimulation

Wanting Chen¹, Rui Chen¹, Qinghua He^1,2,3,4,5

¹Faculty of Psychology, Southwest University, ²Key Laboratory of Cognition and Personality, Ministry of Education, Southwest University, ³Southwest University Branch, Collaborative Innovation Center of Assessment toward Basic Education Quality, Beijing Normal University, ⁴Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, ⁵Chongqing Collaborative Innovation Center for Brain Science

Presented here is a protocol to achieve higher accuracy in determination of stimulation location combining a 3D digitizer with high-definition transcranial direct current stimulation.

Bioengineering

Microfluidic Model to Mimic Initial Event of Neovascularization

Ping Zhao¹, Xing Zhang¹, Xiao Liu¹, Li Wang⁴, Haoran Su¹, Liyi Wang¹, Dongrui Zhang¹, Xiaoyan Deng³, Yubo Fan^1,2

¹Beijing Advanced Innovation Centre for Biomedical Engineering, Key Laboratory for Biomechanics and Mechanobiology of Chinese Education Ministry, School of Biological Science and Medical Engineering, Beihang University, ²School of Engineering Medicine, Beihang University, ³Artificial Intelligence Key Laboratory of Sichuan Province, School of Automation and Information Engineering, Sichuan University of Science and Engineering, ⁴Beijing Research Center of Urban System Engineering

Here, we provide a microfluidic chip and an automatically controlled, highly efficient circulation microfluidic system that recapitulates the initial microenvironment of neovascularization, allowing endothelial cells (ECs) to be stimulated by high luminal shear stress, physiological level of transendothelial flow, and various vascular endothelial growth factor (VEGF) distribution simultaneously.

Biology

Microsurgical Obstruction of Testes Fusion in Spodoptera litura

Xiaolin He^*1, Qianqian Ma^*1, Baozhu Jian¹, Yucheng Liu¹, Qiong Wu¹, Meixin Chen¹, Qili Feng¹, Ping Zhao², Lin Liu¹

¹South China Normal University-Shipai Campus: South China Normal University, ²Southwest University

Aluminum foil was microsurgically inserted between the testes of Spodoptera litura to obstruct the fusion of testis. The procedure includes freezing, fixing, disinfection, incision, placing the barrier, suturing, postoperative feeding, and inspection. This approach provides a method to interfere with tissue formation.

Immunology and Infection

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus

Chengwen Zhang^1,2, Chuanfei Xu², Fumei Luo^2,3, Yu Zuo², Ping Luo², Ping Cheng², Weijun Zhang², Si Sun², Hao Zeng², Quanming Zou²

¹Medical Research Institute, Southwest University, ²National Engineering Research Center of Immunological Products, Third Military Medical University, ³School of Pharmacy, University of South China

Staphylococcus aureus (S. aureus) has the capability to disseminate throughout the body, causing persistent and recurrent infections. To better understand these processes, this study establishes an intracellular infection model for S. aureus. This model will provide a crucial foundation for investigating the mechanisms behind intracellular infections.