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Tokyo Metropolitan University

3 ARTICLES PUBLISHED IN JoVE

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Biology

Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus
Ke Ren 1, Yidan Dai 1, Kai Yi 2, Masanobu Kinoshita 1, Masahiro Itoh 2, Ichiro Sakata 3, Takafumi Sakai 3, Shuang-Qin Yi 1
1Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 2Department of Anatomy, Tokyo Medical University, 3Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University

A whole-mount immunohistochemical approach, to visualize neurofilament protein expression in the extrahepatic biliary tract in Suncus murinus. is presented here. This protocol can be used to analyze the innervation of all visceral organs in S. murinus or other species.

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Research

Near-Infrared Temperature Measurement Technique for Water Surrounding an Induction-heated Small Magnetic Sphere
Naoto Kakuta 1, Keisuke Nishijima 1, Van Cuong Han 1, Yuki Arakawa 1, Katsuya Kondo 2, Yukio Yamada 3
1Department of Mechanical Engineering, Tokyo Metropolitan University, 2Department of Electrical and Electronic Engineering, Tottori University, 3Brain Science Inspired Life Support Research Center, The University of Electro-Communications

A technique utilizing wavelengths of 1150 and 1412 nm to measure the temperature of water surrounding an induction-heated small magnetic sphere is presented.

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Environment

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Megumi Kuroiwa 1, Keitaro Fukushima 2,3, Kazuma Hashimoto 2, Yukiko Senga 4, Tsubasa Sato 4, Chie Katsuyama 5, Yuichi Suwa 1
1Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, 2Faculty & Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, 3Center for Ecological Research, Kyoto University, 4Department of Chemistry, Faculty of Science, Toho University, 5Graduate School of Integrated Arts and Sciences, Hiroshima University

A series of methods to determine the potential DNRA rate based on 14NH4+/15NH4+ analyses is provided in detail. NH4+ is converted into N2O via several steps and analyzed using quadrupole gas chromatography–mass spectrometry.

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