A Simple Chelex Protocol for DNA Extraction from Anopheles spp.Mulenga Musapa 1, Taida Kumwenda 1, Mtawa Mkulama 1, Sandra Chishimba 1, Douglas E. Norris 2, Philip E. Thuma 1, Sungano Mharakurwa 1
1Malaria Institute at Macha, 2Department of Molecular Microbiology & Immunology, Johns Hopkins Bloomberg School of Public Health
A rapid and affordable way to extract quality malaria parasite and vector DNA from mosquito specimens is described. Capitalizing on chelating properties of Chelex resin, the simple method enables genotyping of malaria parasites in mosquito mid-gut and salivary gland phases, as well as molecular identification of the Anopheles sibling species by PCR.
