Humanitas Clinical and Research Center

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions

Achille Broggi^1,2, Clara Cigni¹, Ivan Zanoni^1,2,3, Francesca Granucci^1,3

¹Department of Biotechnology and Biosciences, University of Milano-Bicocca, ²Boston Children's Hospital, Division of Gastroenterology, Harvard Medical School, ³Humanitas Clinical and Research Center

The skin is home to a complex immune cell network. We describe an efficient methodology for the digestion of mouse skin, from different parts of the animal's body, in order to obtain a single-cell suspension and analyze the different leukocyte populations resident in the skin by flow cytometry.

Immunology and Infection

Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses

William Santus¹, Francesca Mingozzi¹, Marina Vai¹, Francesca Granucci^*1, Ivan Zanoni^*1,2

¹Department of Biotechnology and Biosciences, University of Milano-Bicocca, ²Harvard Medical School and Division of Gastroenterology, Boston Children's Hospital

Here we describe a protocol that allows histological and molecular analysis of skin samples after Candida albicans intradermal injection. This protocol maintains the structural integrity of the skin and allows for the localization of tissue-resident or newly recruited immune cells as well as the pathogen distribution.