University Hospital Regensburg

3 ARTICLES PUBLISHED IN JoVE

Medicine

The Influence of Liver Resection on Intrahepatic Tumor Growth

Hannes H. Brandt¹, Valérie Nißler², Roland S. Croner³

¹Friedrich-Alexander-University Erlangen-Nürnberg (FAU), ²Department of Surgery, University Hospital Regensburg, ³Department of Surgery, University Hospital Erlangen

A high incidence of tumor recurrence after resection of liver metastases remains an unsolved problem. The illustrated mouse model may be useful to investigate the reasons for such recurrences. It combines a liver resection model with intrahepatic tumor cell injection for the first time.

Cancer Research

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

Florian Stritzke^1,2, Hendrik Poeck^1,2,3,4, Simon Heidegger^1,2

¹Department of Medicine III, School of Medicine, Technical University of Munich, ²Center for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, ³Department of Internal Medicine III, University Hospital Regensburg, ⁴National Centre for Tumor Diseases WERA

This manuscript describes how to assess in vivo immunogenicity of tumor cell-derived extracellular vesicles (EVs) using flow cytometry. EVs derived from tumors undergoing treatment-induced immunogenic cell death seem particularly relevant in tumor immunosurveillance. This protocol exemplifies the assessment of oxaliplatin-induced immunostimulatory tumor EVs but can be adapted to various settings.

Bioengineering

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

Ana Luísa Graça¹, Niklas Kroner-Weigl², Viviana Reyes Alcaraz¹, Sigrid Müller-Deubert¹, Maximilian Rudert³, Denitsa Docheva¹

¹Department of Musculoskeletal Tissue Regeneration, Orthopaedic Hospital König-Ludwig-Haus, University of Würzburg, ²Laboratory for Experimental Trauma Surgery, Department of Trauma Surgery, University Hospital Regensburg, ³Department of Orthopaedics, Orthopaedic Hospital König-Ludwig-Haus, University of Würzburg

Herein, we demonstrate a three-step organoid model (two-dimensional [2D] expansion, 2D stimulation, three-dimensional [3D] maturation) offering a promising tool for tendon fundamental research and a potential scaffold-free method for tendon tissue engineering.