Haukeland University Hospital

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives

Kristina Xiao Liang^*1,2, Anbin Chen^*1,2,3,4, Cecilie Katrin Kristiansen^1,2, Laurence A. Bindoff^1,2

¹Department of Clinical Medicine (K1), University of Bergen, ²Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases, Haukeland University Hospital, ³Department of Neurosurgery, Qilu Hospital and Institute of Brain and Brain-Inspired Science, Cheeloo College of Medicine, Shandong University, ⁴Shandong Key Laboratory of Brain Function Remodeling

This study reports a novel approach to measure multiple mitochondrial functional parameters based on flow cytometry and double staining with two fluorescent reporters or antibodies to detect changes in mitochondrial volume, mitochondrial membrane potential, reactive oxygen species level, mitochondrial respiratory chain composition, and mitochondrial DNA.

Neuroscience

Application of Flow Cytometric Analysis for Measuring Multiple Mitochondrial Parameters in 3D Brain Organoids

Sharika Marjan^1,2, Tsering Yangzom^1,2, Cecilie Katrin Kristiansen^1,3, Anbin Chen⁴, Laurence A. Bindoff^1,5,6, Kristina Xiao Liang^1,3

¹Department of Clinical Medicine (K1), University of Bergen, ²Centre for International Health, Department of Global Public Health and Primary Care, University of Bergen, ³Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases, Haukeland University Hospital, ⁴Department of Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, ⁵Department of Neurology, Haukeland University Hospital, ⁶National Advisory Unit for Congenital Metabolic Diseases, Oslo University Hospital

This protocol reports a unique method of using a streaming cytometer and multiple antibodies for simultaneous assessment of multiple mitochondrial functional parameters, including changes in mitochondrial volume, amounts of the mitochondrial respiratory chain (MRC) complex subunits, and mitochondrial DNA (mtDNA) replication.