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Max Planck Institute for Terrestrial Microbiology

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells
Jan Heering 1, Alejandra Alvarado 1, Simon Ringgaard 1
1Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology

This protocol enables single cell microscopy of the differentially distinct Vibrio parahaemolyticus swimmer and swarmer cells. The method produces a population of swarmer cells easily available for single cell analysis and covers preparation of cell cultures, induction of swarmer differentiation, sample preparation, and image analysis.

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Biochemistry

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus
Dominik Schumacher 1, Lotte Søgaard-Andersen 1
1Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology

Bacterial cells are spatially highly organized. To follow this organization over time in slow growing Myxococcus xanthus cells, a set-up for fluorescence live-cell imaging with high spatiotemporal resolution over several generations was developed. Using this method, spatiotemporal dynamics of important proteins for chromosome segregation and cell division could be determined.

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