Mass Spectrometric Analysis of Glycosphingolipid AntigensAlexandra Bili Yin 1, David Hawke 2, Dapeng Zhou 3,4
1Undergraduate Program, Rice University , 2Proteomics Facility, Department of Pathology, University of Texas MD Anderson Cancer Center , 3Department of Melanoma Medical Oncology, University of Texas MD Anderson Cancer Center , 4University of Texas Graduate School of Biological Sciences at Houston
A specific and sensitive method to gain insight into the expression profile of glycosphingolipid antigens in immune organs and cells is described. The method takes advantage of the ion trap mass spectrometry allowing step-wise fragmentation of glycosphingolipid molecules for structural analysis in comparison to chemically synthesized standards.