JoVE Logo

Sign In

University of Texas Graduate School of Biological Sciences at Houston

1 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Mass Spectrometric Analysis of Glycosphingolipid Antigens
Alexandra Bili Yin 1, David Hawke 2, Dapeng Zhou 3,4
1Undergraduate Program, Rice University , 2Proteomics Facility, Department of Pathology, University of Texas MD Anderson Cancer Center , 3Department of Melanoma Medical Oncology, University of Texas MD Anderson Cancer Center , 4University of Texas Graduate School of Biological Sciences at Houston

A specific and sensitive method to gain insight into the expression profile of glycosphingolipid antigens in immune organs and cells is described. The method takes advantage of the ion trap mass spectrometry allowing step-wise fragmentation of glycosphingolipid molecules for structural analysis in comparison to chemically synthesized standards.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved