Improving the Success Rate of Protein Crystallization by Random Microseed Matrix ScreeningMarisa Till 1, Alice Robson 1, Matthew J. Byrne 1, Asha V. Nair 1, Stefan A. Kolek 2, Patrick D. Shaw Stewart 2, Paul R. Race 1
1School of Biochemistry, University of Bristol, 2Douglas Instruments
Here we describe a general method for random microseed matrix screening. This technique is shown to significantly increase the success rate of protein crystallization screening experiments, reduce the need for optimization, and provide a reliable supply of crystals for data collection and ligand-soaking experiments.
