University Medical Center Mainz

3 ARTICLES PUBLISHED IN JoVE

Medicine

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation

J. Moritz Kaths^1,2, Vinzent N. Spetzler¹, Nicolas Goldaracena¹, Juan Echeverri¹, Kristine S. Louis¹, Daniel B. Foltys³, Mari Strempel⁴, Paul Yip⁵, Rohan John⁵, Istvan Mucsi¹, Anand Ghanekar¹, Darius Bagli^6,7, Lisa Robinson², Markus Selzner¹

¹Multi Organ Transplant Program, Department of Surgery, Toronto General Hospital, ²Division of Nephrology, The Hospital for Sick Children, Toronto, ³Department of General, Visceral & Transplant Surgery, University Medical Center Mainz, ⁴Department of Abdominal, Vascular & Transplant Surgery, Merheim Medical Center Cologne, ⁵Laboratory Medicine & Pathobiology, Toronto General Hospital, ⁶Departments of Surgery (Urology) & Physiology, The Hospital for Sick Children, Toronto, ⁷Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto

The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.

Immunology and Infection

Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls

Jeremy Lagrange¹, Sabine Kossmann^1,2, Klytaimnistra Kiouptsi¹, Philip Wenzel^1,2,3

¹Center for Thrombosis and Hemostasis Mainz, University Medical Center Mainz, ²Center for Cardiology, University Medical Center Mainz, ³German Center for Cardiovascular Research (DZHK)

This manuscript describes the use of transgenic reporter mice and different administration routes of fluorescent dyes in angiotensin II-induced hypertension using intravital video microscopy of blood vessels to evaluate the activation of immune cells and their ability to roll and adhere to the endothelium.

Neuroscience

Analysis of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage with High Frequency Transcranial Duplex Ultrasound

Axel Neulen^*1, Michael Molitor^*2,3,4, Michael Kosterhon^*1, Tobias Pantel¹, Susanne H. Karbach^2,3,4, Philip Wenzel^2,3,4, Thomas Gaul⁵, Florian Ringel¹, Serge C. Thal⁵

¹Department of Neurosurgery, University Medical Center of the Johannes Gutenberg-University of Mainz, ²Center for Cardiology - Cardiology I, University Medical Center of the Johannes Gutenberg-University of Mainz, ³Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University of Mainz, ⁴German Center for Cardiovascular Research (DZHK) - Partner site Rhine-Main, ⁵Department of Anesthesiology, University Medical Center of the Johannes Gutenberg-University of Mainz

The aim of this manuscript is to present a sonography-based method that allows in vivo imaging of blood flow in cerebral arteries in mice. We demonstrate its application to determine changes in blood flow velocities associated with vasospasm in murine models of subarachnoid hemorrhage (SAH).