Yokohama City University

2 ARTICLES PUBLISHED IN JoVE

Medicine

Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells

Ran-Ran Zhang^*1, Yun-Wen Zheng^*1,2,3, Hideki Taniguchi¹

¹Department of Regenerative Medicine, Graduate School of Medicine, Yokohama City University, ²Department of Advanced Gastroenterological Surgical Science and Technology, Faculty of Medicine, University of Tsukuba, ³Regenerative Medicine Research Center, Jiangsu University Hospital

Here, we present a novel humanized mouse liver model generated in Alb-toxin receptor mediated cell knockout (TRECK)/SCID mice following the transplantation of immature and expandable human hepatic stem cells.

Medicine

Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy

Eriko Katsuta¹, Masanori Oshi¹, Omar M. Rashid^2,3,4,5, Kazuaki Takabe^1,6,7,8,9,10

¹Breast Surgery, Department of Surgical Oncology, Roswell Park Comprehensive Cancer Center, ²Holy Cross Hospital Michael and Dianne Bienes Comprehensive Cancer Center, ³Department of Surgery, Massachusetts General Hospital, ⁴Department of Surgery, University of Miami Miller School of Medicine, ⁵Department of Surgery, Nova Southeastern University School of Medicine, ⁶Department of Surgery, University at Buffalo Jacobs School of Medicine and Biomedical Sciences, ⁷Department of Breast Surgery and Oncology, Tokyo Medical University, ⁸Department of Surgery, Yokohama City University, ⁹Department of Surgery, Niigata University Graduate School of Medical and Dental Sciences, ¹⁰Department of Surgery, Fukushima Medical University

We introduce a murine orthotopic breast cancer model and radical mastectomy model with bioluminescence technology to quantify the tumor burden to mimic human breast cancer progression.