Imaging Initial Ca2+ Microdomains in Primary T CellsFynn Gerlach *1, Franziska Möckl *1, Dejan Kovacevic 1,2,3, Valerie J. Brock 1, Riekje Winzer 4, Lina Meyer 2,3, David Lohr 2,3, Lena-Marie Woelk 2,3, Eva Tolosa 4, René Werner 2,3, Björn-Philipp Diercks 1
1The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Centre Hamburg-Eppendorf, 2Department of Applied Medical Informatics, University Medical Centre Hamburg-Eppendorf, 3Department of Computational Neuroscience, University Medical Centre Hamburg-Eppendorf, 4Department of Immunology, University Medical Centre Hamburg-Eppendorf
Here, we provide a comprehensive protocol to resolve initial local Ca2+ signals, known as Ca2+ microdomains, in primary murine and human T cells using fluorescence microscopy. This protocol serves as a valuable resource for researchers examining Ca2+ signaling pathways within immune cells and to further unravel their function.
