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German Center for Neurodegenerative Diseases

6 ARTICLES PUBLISHED IN JoVE

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The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson's Disease
Lena F. Burbulla 1,2, Rejko Krüger 1,2
1DZNE, German Center for Neurodegenerative Diseases, 2Department of Neurodegenerative Diseases, Laboratory of Functional Neurogenomics, Hertie Institute for Clinical Brain Research

Fibroblasts from patients carrying mutations in Parkinson's disease-causing genes represent an easily accessible ex vivo model to study disease-associated phenotypes. Live cell imaging gives the opportunity to study morphological and functional parameters in living cells. Here we describe the preparation of human fibroblasts and subsequent monitoring of mitochondrial phenotypes .

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Developmental Biology

Imaging Subcellular Structures in the Living Zebrafish Embryo
Peter Engerer 1, Gabriela Plucinska 1,2, Rachel Thong 1,3, Laura Trovò 1, Dominik Paquet 4,5,6, Leanne Godinho 1
1Institute of Neuronal Cell Biology, Technische Universität München, 2Cell Biology, Department of Biology, Faculty of Science, Utrecht University, 3Faculty of Biology, Ludwig-Maximilians-Universität-München, 4Adolf-Butenandt-Institute, Biochemistry, Ludwig-Maximilians-Universität-München, 5German Center for Neurodegenerative Diseases, 6Laboratory of Brain Development and Repair, The Rockefeller University

Imaging the dynamic behavior of organelles and other subcellular structures in vivo can shed light on their function in physiological and disease conditions. Here, we present methods for genetically tagging two organelles, centrosomes and mitochondria, and imaging their dynamics in living zebrafish embryos using wide-field and confocal microscopy.

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Neuroscience

Non-restraining EEG Radiotelemetry: Epidural and Deep Intracerebral Stereotaxic EEG Electrode Placement
Anna Papazoglou 1, Andreas Lundt 1, Carola Wormuth 1, Dan Ehninger 2, Christina Henseler 1, Julien Soós 1, Karl Broich 1, Marco Weiergräber 1
1Department of Neuropsychopharmacology, Federal Institute for Drugs and Medical Devices (Bundesinstitut für Arzneimittel und Medizinprodukte, BfArM), 2Molecular and Cellular Cognition Lab, German Center for Neurodegenerative Diseases (Deutsches Zentrum für Neurodegenerative Erkrankungen, DZNE)

Non-restraining EEG radiotelemetry is a valuable methodological approach to record in vivo long-term electroencephalograms from freely moving rodents. This detailed protocol describes stereotaxic epidural and deep intracerebral electrode placement in different brain regions in order to obtain reliable recordings of CNS rhythmicity and CNS related behavioral stages.

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Behavior

Automatic Detection of Highly Organized Theta Oscillations in the Murine EEG
Ralf Müller 1, Anna Papazoglou 2, Julien Soos 2, Andreas Lundt 2, Carola Wormuth 2, Christina Henseler 2, Dan Ehninger 3, Karl Broich 4, Marco Weiergräber 2
1Department of Psychiatry and Psychotherapy, University of Cologne, 2Department of Neuropsychopharmacology, Federal Institute for Drugs and Medical Devices, 3Molecular and Cellular Cognition Lab, German Center for Neurodegenerative Diseases, 4Federal Institute for Drugs and Medical Devices

Theta activity in the hippocampus is related to specific cognitive and behavioral stages. Here, we describe an analytical method to detect highly-organized theta oscillations within the hippocampus using a time-frequency (i.e., wavelet analysis)-based approach.

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Neuroscience

Visualization of Cortical Modules in Flattened Mammalian Cortices
Simon M. Lauer *1, Undine Schneeweiß 1, Michael Brecht 1,2,3, Saikat Ray *1
1Bernstein Center for Computational Neuroscience, Humboldt University of Berlin, 2NeuroCure Cluster of Excellence, 3German Center for Neurodegenerative Diseases

This article describes a detailed methodology to obtain flattened tangential sections from mammalian cortices and visualize cortical modules using histochemical and immunohistochemical methods.

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Neuroscience

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice
Andreas Lundt 1,2, Julien Soos 1, Christina Henseler 1, Muhammad Imran Arshaad 1, Ralf Müller 3, Dan Ehninger 4, Jürgen Hescheler 5, Agapios Sachinidis 5, Karl Broich 6, Carola Wormuth 1,7, Anna Papazoglou 1, Marco Weiergräber 1
1Experimental Neuropsychopharmacology, Federal Institute for Drugs and Medical Devices, 2KBRwyle GmbH, 3Cognitive Neurophysiology, Department of Psychiatry and Psychotherapy, Faculty of Medicine, University of Cologne, 4Molecular and Cellular Cognition, German Center for Neurodegenerative Diseases (DZNE), 5Institute of Neurophysiology, Faculty of Medicine, University of Cologne, 6Federal Institute for Drugs and Medical Devices (BfArM), 7Thescon GmbH

Brainstem evoked response audiometry is an important tool in clinical neurophysiology. Nowadays, brainstem evoked response audiometry is also applied in the basic science and preclinical studies involving both pharmacological and genetic animal models. Here we provide a detailed description of how auditory brainstem responses can be successfully recorded and analyzed in mice.

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