Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)Carl White 1, Alex Nixon 1, Neil A. Bradbury 1
1Department of Physiology and Biophysics, Chicago Medical School
Here, we present a protocol to determine the orientation and topology of integral membrane proteins in living cells. This simple protocol relies on selective protease sensitivity of chimeras between the protein of interest and GFP.
