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Lynntech

3 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
Caroline Gravel *1, Changgui Li *2, Junzhi Wang 2, Anwar M Hashem 1,3,4, Bozena Jaentschke 1, Gary Van Domselaar 5, Runtao He 5, Xuguang Li 1,3
1Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, HPFB, Health canada, 2National Institute for the Control of Pharmaceutical and Biological Products, The State Food and Drug Administration, Beijing, 3Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 4Microbiology Department, Faculty of Medicine, King Abdulaziz University, 5National Microbiology Laboratory, Public Health Agency of Canada

A simple slot blot method was developed for the quantification of influenza viral hemagglutinin and neuraminidase using universal antibodies targeting their most conserved sequences identified through bioinformatics analyses. This innovative approach may provide a useful alternative to quantitative determination of all viral hemagglutinin and neuraminidase.

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Engineering

Fabrication of VB2/Air Cells for Electrochemical Testing
Jessica Stuart 1, Ruben Lopez 2, Jason Lau 1, Xuguang Li 2, Mahesh Waje 2, Matthew Mullings 2, Christopher Rhodes 2, Stuart Licht 1
1Department of Chemistry, The George Washington University, 2Lynntech

A protocol is presented to study multi-electron metal/air battery systems by using previous technology developed for the zinc/air cell. Electrochemical testing is then performed on fabricated batteries to evaluate performance.

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Engineering

Non-aqueous Electrode Processing and Construction of Lithium-ion Coin Cells
Malcolm Stein IV 1, Chien-Fan Chen 1, Daniel J. Robles 1, Christopher Rhodes 2, Partha P. Mukherjee 1
1Department of Mechanical Engineering, Texas A&M University, 2Department of Chemistry and Biochemistry, Texas State University

Non-aqueous electrode processing is central to the construction of coin cells and the evaluation of new electrode chemistries for lithium-ion batteries. A step-by-step guide to the basic practices needed as an electrochemical engineer working with batteries in an academic experimental setting is furnished.

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