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University of Virginia Health Sciences Center

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells
M. Brittany Johnson 1, Alison K. Criss 1
1Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health Sciences Center

Central to the field of bacterial pathogenesis is the ability to define if and how microbes survive after exposure to eukaryotic cells. This article outlines protocols for the use of fluorescent dyes that reveal the viability of individual bacteria inside and associated with host cells.

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