Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian CellsM. Brittany Johnson 1, Alison K. Criss 1
1Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health Sciences Center
Central to the field of bacterial pathogenesis is the ability to define if and how microbes survive after exposure to eukaryotic cells. This article outlines protocols for the use of fluorescent dyes that reveal the viability of individual bacteria inside and associated with host cells.
