University of Utah Health Sciences Center

2 ARTICLES PUBLISHED IN JoVE

Medicine

Detecting Abnormalities in Choroidal Vasculature in a Mouse Model of Age-related Macular Degeneration by Time-course Indocyanine Green Angiography

Sandeep Kumar¹, Zachary Berriochoa¹, Alex D. Jones¹, Yingbin Fu^1,2

¹Department of Ophthalmology & Visual Sciences, University of Utah Health Sciences Center, ²Department of Neurobiology & Anatomy, University of Utah Health Sciences Center

Indocyanine Green Angiography (or ICGA) performed by tail vein injection provides high quality ICGA time course images to characterize abnormalities in mouse choroid.

Biology

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Sandeep Kumar^*1, Aitor Andueza^*1, Nicolas Villa-Roel¹, Juyoung Kim¹, Dong-Won Kang¹, Hanjoong Jo^1,2

¹Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, ²Division of Cardiology, Georgia Institute of Technology and Emory University

We present a method for isolating endothelial cells and nuclei from the lumen of mouse carotid arteries exposed to stable or disturbed flow conditions to perform single-cell omics experiments.