Boston Children's Hospital and Harvard Medical School

5 ARTICLES PUBLISHED IN JoVE

Biology

Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland

Silva Krause¹, Amy Brock², Donald E. Ingber^1,2,3

¹Vascular Biology Program, Department of Surgery, Boston Children's Hospital and Harvard Medical School, ²Wyss Institute for Biologically Inspired Engineering, Harvard University, ³Harvard School of Engineering and Applied Sciences

A protocol for the non-invasive intraductal delivery of aqueous reagents to the mouse mammary gland is described. The method takes advantage of localized injection into the nipples of mammary glands targeting mammary ducts specifically. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents and small molecules.

Medicine

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

Tadanori Mammoto¹, Akiko Mammoto¹

¹Vascular Biology Program, Department of Surgery, Boston Children's Hospital and Harvard Medical School

Recapitulation of the organ-specific microenvironment, which stimulates local angiogenesis, is indispensable for successful regeneration of damaged tissues. This report demonstrates a novel method to implant fibrin gels on the lung surface of living mouse in order to explore how the lung-specific microenvironment modulates angiogenesis and alveolar regeneration in adult mouse.

Bioengineering

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Hyun Jung Kim¹, Jaewon Lee¹, Jin-Ha Choi¹, Anthony Bahinski², Donald E. Ingber^2,3,4

¹Department of Biomedical Engineering, The University of Texas at Austin, ²Wyss Institute for Biologically Inspired Engineering at Harvard University, ³Vascular Biology Program, Boston Children's Hospital, Harvard Medical School, ⁴John A. Paulson School of Engineering and Applied Sciences, Harvard University

We describe an in vitro protocol to co-culture gut microbiome and intestinal villi for an extended period using a human gut-on-a-chip microphysiological system.

Bioengineering

Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips

Richard Novak^*1, Meredyth Didier^*1,2, Elizabeth Calamari¹, Carlos F Ng¹, Youngjae Choe¹, Susan L Clauson¹, Bret A Nestor¹, Jefferson Puerta¹, Rachel Fleming¹, Sasan J Firoozinezhad¹, Donald E Ingber^1,3,4

¹Wyss Institute for Biologically Inspired Engineering, Harvard University, ²Apple, Inc, ³Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, ⁴Vascular Biology Program and Department of Surgery, Boston Children's Hospital and Harvard Medical School

Here, we present a protocol that describes the fabrication of stretchable, dual channel, organ chip microfluidic cell culture devices for recapitulating organ-level functionality in vitro.

Bioengineering

Modeling Healthy and Dysbiotic Vaginal Microenvironments in a Human Vagina-on-a-Chip

Aakanksha Gulati¹, Alicia Jorgenson¹, Abidemi Junaid¹, Donald E. Ingber^1,2,3

¹Wyss Institute for Biologically Inspired Engineering, Harvard University, ²Vascular Biology Program and Department of Surgery, Boston Children's Hospital and Harvard Medical School, ³Harvard John A. Paulson School of Engineering and Applied Sciences

This article describes a protocol for creating a microfluidic vagina-on-a-chip (Vagina Chip) culture device that enables the study of human host interactions with a living vaginal microbiome under microaerophilic conditions. This chip can be used as a tool to investigate vaginal diseases as well as to develop and test potential therapeutic countermeasures.