Institute for Adriatic Crops and Karst Reclamation

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Adi Kliot^1,2, Svetlana Kontsedalov¹, Galina Lebedev¹, Marina Brumin¹, Pakkianathan Britto Cathrin¹, Julio Massaharu Marubayashi¹, Marisa Skaljac^1,3, Eduard Belausov⁴, Henryk Czosnek², Murad Ghanim¹

¹Department of Entomology, Volcani Center, ²Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, ³Department of Applied Sciences, Institute for Adriatic Crops and Karst Reclamation, ⁴The Institute of Plant Sciences, Volcani Center

We describe here a simple fluorescence in situ hybridization (FISH) method for the localization of viruses and bacteria in insect and plant tissues. This protocol can be extended for the visualization of mRNA in whole mount and microscopic sections.