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Naval Research Laboratory

12 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection
Kelly L. Robertson 1, Gary J. Vora 1
1Center for Bio/Molecular Science and Engineering, Naval Research Laboratory

A novel high-throughput method is described that enables the detection and relative quantitation of small RNA and mRNA expression from single bacterial cells using locked nucleic acid probes and flow cytometry-fluorescence in situ hybridization.

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Chemistry

A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
Jake Fontana 1, Christopher Spillmann 1, Jawad Naciri 1, Banahalli R. Ratna 1
1Naval Research Laboratory

A simple, robust and scalable technique to functionalize and self-assemble macroscopic nanoparticle-ligand monolayer films onto template-free substrates is described in this protocol.

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Engineering

Formation of Thick Dense Yttrium Iron Garnet Films Using Aerosol Deposition
Scooter D. Johnson 1, Evan R. Glaser 2, Fritz J. Kub 1, Charles R. Eddy, Jr. 1
1Power Electronics Branch, Naval Research Laboratory, 2Physics of Electronic Materials Branch, Naval Research Laboratory

This report describes the use of a custom-built system to perform aerosol deposition of thick films of yttrium iron garnet onto sapphire substrates at RT. The deposited films are characterized using scanning electron microscopy, profilometry, and ferromagnetic resonance to give a representative overview of the capabilities of the technique.

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Bioengineering

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
Deepa Raghu 1, Joseph A. Christodoulides 1, James B. Delehanty 2, Jeff M. Byers 1, Marc P. Raphael 1
1Materials Science and Technology, Naval Research Laboratory, 2Center for Bio/Molecular Science and Engineering, Naval Research Laboratory

Inter-cellular communication is critical for controlling various physiological activities within and outside the cell. This paper describes a protocol for measuring the spatio-temporal nature of single cell secretions. To achieve this, a multidisciplinary approach is used which integrates label-free nanoplasmonic sensing with live cell imaging.

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Engineering

Laser-induced Forward Transfer of Ag Nanopaste
Eric Breckenfeld 1, Heungsoo Kim 2, Raymond C. Y. Auyeung 2, Alberto Piqué 2
1National Research Council Research Associates Program, Naval Research Laboratory, 2Materials Science and Technology Division, Naval Research Laboratory

We demonstrate the use of the Laser-induced forward transfer technique (LIFT) for the printing of high-viscosity Ag paste. This technique offers a simple, low temperature, robust process for non-lithographically printing microscale 2D and 3D structures.

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Engineering

Fabrication of Fully Solution Processed Inorganic Nanocrystal Photovoltaic Devices
Troy K. Townsend 1, Dario Durastanti 1, William B. Heuer 2, Edward E. Foos 3, Woojun Yoon 4, Joseph G. Tischler 4
1Department of Chemistry and Biochemistry, St. Mary's College of Maryland, 2Department of Chemistry, U.S. Naval Academy, 3NSWC Indian Head EOD Technology Division, 4Electronics and Devices Division, Naval Research Laboratory

This protocol describes the synthesis and solution deposition of inorganic nanocrystals layer by layer to produce thin film electronics on non-conductive surfaces. Solvent-stabilized inks can produce complete photovoltaic devices on glass substrates via spin and spray coating following post-deposition ligand exchange and sintering.

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Biochemistry

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
Nathan J. Alves 1,2, Kendrick B. Turner 1, Scott A. Walper 1
1Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, 2Department of Emergency Medicine, Indiana University of School of Medicine

A protocol for the production, purification, and use of enzyme packaged outer membrane vesicles (OMV) providing for enhanced enzyme stability for implementation across diverse applications is presented.

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Education

Characterizing Electron Transport through Living Biofilms
Matthew Yates 1, Sarah Strycharz-Glaven 1, Joel Golden 1, Jared Roy 1,2, Stanislav Tsoi 3, Jeffrey Erickson 1, Mohamed El-Naggar 4, Scott Calabrese Barton 5, Leonard Tender 1
1Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, 2George Mason University, 3Chemistry Division, Naval Research Laboratory, 4Departments of Physics, Biological Sciences, and Chemistry, University of Southern California, 5Department of Chemical Engineering and Materials Science, Michigan State University

A protocol for measuring electrical conductivity of living microbial biofilms under physiologically relevant conditions is presented.

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Engineering

Plasma-assisted Molecular Beam Epitaxy of N-polar InAlN-barrier High-electron-mobility Transistors
Matthew T. Hardy 1, David F. Storm 2, D. Scott Katzer 2, Brian P. Downey 2, Neeraj Nepal 2, David J. Meyer 2
1NRC Postdoctoral Scholar, Naval Research Laboratory, 2Electronics Science and Technology Division, Naval Research Laboratory

Molecular beam epitaxy is used to grow N-polar InAlN-barrier high-electron-mobility transistors (HEMTs). Control of the wafer preparation, layer growth conditions and epitaxial structure results in smooth, compositionally homogeneous InAlN layers and HEMTs with mobility as high as 1,750 cm2/V∙sec.

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Bioengineering

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier
Okhil K. Nag 1, Jawad Naciri 1, Eunkeu Oh 2,3, Christopher M. Spillmann 1, James B. Delehanty 1
1Center for Biomolecular Science and Engineering, Naval Research Laboratory, 2Optical Sciences Division, Naval Research Laboratory, 3Sotera Defense Solutions

A liquid crystal nanoparticle (LCNP) nanocarrier is exploited as a vehicle for the controlled delivery of a hydrophobic cargo to the plasma membrane of living cells.

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Engineering

Atomic Layer Deposition of Vanadium Dioxide and a Temperature-dependent Optical Model
Marc Currie 1, Michael A. Mastro 1, Virginia D. Wheeler 1
1Naval Research Laboratory

Thin films (100-1000 Å) of vanadium dioxide (VO2) were created by atomic-layer deposition (ALD) on sapphire substrates. Following this, the optical properties were characterized through the metal-insulator transition of VO2. From the measured optical properties, a model was created to describe the tunable refractive index of VO2.

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Chemistry

Deposition of Porous Sorbents on Fabric Supports
Brandy J. Johnson 1, Brian J. Melde 1, Martin H. Moore 1, Jenna R. Taft 2
1Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, 2Department of Chemistry, University of Vermont

This report details a microwave-initiated approach for deposition of porphyrin functionalized porous organosilicate sorbents on a cotton fabric and demonstrates reduction in 2-chloroethyl ethyl sulfide (CEES) transport through the fabric resulting from this treatment.

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