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Anhui medical university

7 ARTICLES PUBLISHED IN JoVE

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Biology

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
Juan Du 1,2, Hendrik Rietman 1, Vivianne G. A. A. Vleeshouwers 1
1Wageningen UR Plant Breeding, Wageningen University, 2Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Ministry of Education, National Centre for Vegetable Improvement (Central China), Potato Engineering and Technology Research Centre of Hubei Province, Huazhong Agricultural University

Agroinfiltration and PVX agroinfection are routine functional assays for transient ectopic expression of genes in plants. These methods are efficient assays in effectoromics strategies (rapid resistance and avirulence gene discovery) and crucial to modern research in molecular plant pathology. They meet the demand for robust high-throughput functional analysis in plants.

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Medicine

Ultrasonography in Experimental Reproductive Investigations on Rats
Tianjuang Wang 1,2, Lidia Oltra-Rodríguez 3, Nuria García-Carrillo 3, Anibal Nieto 4,5, Yunxia Cao *1,2,6, María L. Sánchez-Ferrer *4,5
1Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, 2Institute of Reproductive Genetics, Anhui Medical University, 3Centro Experimental en Investigaciones Biomédica (CEIB), Campus Ciencias de la Salud, Universidad de Murcia, 4Department of Obstetrics and Gynecology, 'Virgen de la Arrixaca' University Clinical Hospital, 5Institute for Biomedical Research of Murcia, IMIB-Arrixaca, 6Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs

This manuscript describes the utility of ultrasound performed on female rats to design experimental models for reproductive and gynecological investigation. A step-by-step explanation of how to perform ultrasonographic evaluation is shown.

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Cancer Research

Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro
Jinsen Lu 1, Lele Wu 2, Xiaoke Wang 2, Jinhang Zhu 2, Juan Du 2, Bing Shen 2
1School of Basic Medical Sciences, Anhui Medical University, 2Department of Orthopedics, Anhui Provincial Hospital, Anhui Medical University

Here we present a detailed protocol for the application of rhodamine 123 to identify the mitochondrial membrane potential (MMP) and study CLIC4 knockdown-induced HN4 cell apoptosis in vitro. Under common fluorescence microscope and confocal laser scanning fluorescence microscope, the real-time change of the MMP was recorded.

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Biochemistry

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
Emery Haley *1, Wooyoung Choi *1, Chen Fan 1, Weinan Sun 2,3, Juan Du 1, Wei Lü 1
1Van Andel Institute, 2Vollum Institute, 3Janelia Research Campus

This protocol describes techniques used to determine ion channel structures by cryo-electron microscopy, including a baculovirus system used to efficiently express genes in mammalian cells with minimum effort and toxicity, protein extraction, purification, and quality checking, sample grid preparation and screening, as well as data collection and processing.

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Chemistry

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
Chunli Song *1, Zhanfeng Hou *1,2, Zijun Jiao *1, Zhaodi Liu 3, Chenshan Lian 1,2, Min Zhang 1, Wei Liang 3, Feng Yin 1,2, Zigang Li 1,2
1Pingshan Translational Medicine Center, Shenzhen Bay Laboratory, 2Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, 3Department of Radiation Oncology, the First Affiliated Hospital, Anhui Medical University

This protocol presents the synthesis of cyclic peptides via bisalkylation between cysteine and methionine and the facile thiol-yne reaction triggered by the propargyl sulfonium center.

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Biochemistry

Identification of Peptides of Small Extracellular Vesicles from Bone Marrow-Derived Macrophages
Jiale Cheng 1,2, Jiong Zhu 1,2, Yujiao Liu 2,3, Chen Yang 2,3, Yuehui Zhang 2, Yuchen Liu 2, Chaozhi Jin 2, Jian Wang 1,2
1School of Basic Medical Sciences, Anhui Medical University, 2State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, 3School of Life Sciences, Hebei University

This protocol describes a procedure to isolate small extracellular vesicles from macrophages by differential ultracentrifugation and extract the peptidome for identification by mass spectrometry.

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Medicine

Comparison of Three Clinical Stereoscopic Methods for Measuring Binocular Visual Function During Amblyopic Treatment in Unilateral Amblyopia
Jiafeng Wang 1, Lixia Feng 2, Heting Liu 1, Qianqian Wan 1, Liming Tao 1
1Department of Ophthalmology, Second Affiliated Hospital, Anhui Medical University, 2Department of Ophthalmology, First Affiliated Hospital, Anhui Medical University

Here, we present three common stereopsis measurement methods to measure patients with amblyopia and the comparison of the efficacy of different methods.

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