Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico

4 ARTICLES PUBLISHED IN JoVE

Biology

Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts

Deborah Mattinzoli¹, Piergiorgio Messa², Alessandro Corbelli¹, Masami Ikehata¹, Anna Mondini¹, Cristina Zennaro³, Silvia Armelloni¹, Min Li¹, Laura Giardino¹, Maria Pia Rastaldi¹

¹Renal Research Laboratory, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, ²Department of Nephrology, Dialysis and Renal Transplant, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, ³Renal Physiopathology Laboratory, Department of Medical, Surgical and Health Sciences, University of Trieste

Treatment of primary mouse osteoblasts with retinoic acid produces a homogeneous population of ramified cells bearing morphological and molecular features of osteocytes. The method overcomes the difficulty of obtaining and maintaining primary osteocytes in culture, and can be advantageous to study cells derived from transgenic models.

JoVE Core

Brain Morphology of Cannabis Users With or Without Psychosis: A Pilot MRI Study

Giuseppe Delvecchio^*1, Lucio Oldani^*2, Gian Mario Mandolini², Alessandro Pigoni³, Valentina Ciappolino², Giandomenico Schiena², Matteo Lazzaretti², Elisabetta Caletti², Viviana Barbieri¹, Claudia Cinnante⁴, Fabio Triulzi^1,4, Paolo Brambilla^1,2

¹Department of Pathophysiology and Transplantation, University of Milan, ²Department of Neurosciences and Mental Health, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, ³MoMiLab Research Unit, IMT School for Advanced Studies Lucca, ⁴Neuroradiology Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico

This is a 3T magnetic resonance imaging study aiming to investigate grey matter volume differences between cannabis-induced psychosis patients and non-psychotic chronic cannabis users.

Medicine

Transthoracic Echocardiography to Assess Post-Resuscitation Left Ventricular Dysfunction After Acute Myocardial Infarction and Cardiac Arrest in Pigs

Daria De Giorgio¹, Davide Olivari¹, Francesca Fumagalli¹, Lidia Staszewsky¹, Giuseppe Ristagno^2,3

¹Istituto di Ricerche Farmacologiche Mario Negri IRCCS, ²University of Milan, ³Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico

Transthoracic echocardiography is the first-line diagnostic test for post-resuscitation left ventricular dysfunction and structural changes in a pig model of cardiac arrest.

Biology

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Min Li^*1, Hongkun Qin^*1, Yunfei Long¹, Meiqin Cheng¹, Ling Li¹, Aijun Huang², Nian Wang³, Shuo Duan¹

¹China-USA Citrus Huanglongbing Joint Laboratory, National Navel Orange Engineering Research Center, Gannan Normal University, ²Department of Biological Sciences, Gannan Normal University, ³Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida - Institute of Food and Agricultural Sciences

In this work, a rapid, sensitive, and portable detection method for Candidatus Liberibacter asiaticus based on recombinase polymerase amplification combined with CRISPR-Cas12a was developed.