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Bowling Green State University

4 ARTICLES PUBLISHED IN JoVE

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Biology

Single-Molecule Imaging of Nuclear Transport
Alexander Goryaynov 1, Ashapurna Sarma 1, Jiong Ma 1, Weidong Yang 1,2
1Department of Biology, Bowling Green State University, 2Center for Photochemical Sciences, Bowling Green State University

Single molecule microscopy approch provided novel insights into nuclear transport.

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Engineering

Harvesting Solar Energy by Means of Charge-Separating Nanocrystals and Their Solids
Geoffrey Diederich 1, Timothy O'Connor 1, Pavel Moroz 2,3, Erich Kinder 1, Elena Kohn 2,3, Dimuthu Perera 1, Ryan Lorek 1, Scott Lambright 1, Martene Imboden 3, Mikhail Zamkov 1,2
1Department of Physics, Bowling Green State University, 2The Center for Photochemical Sciences, Bowling Green State University, 3Department of Chemistry, Bowling Green State University

A general strategy for the development of charge-separating semiconductor nanocrystal composites deployable for solar energy production is presented. We show that assembly of donor-acceptor nanocrystal domains in a single nanoparticle geometry gives rise to a photocatalytic function, while bulk-heterojunctions of donor-acceptor nanocrystal films can be used for photovoltaic energy conversion.

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JoVE Core

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
Andrew Ruba 1, Wangxi Luo 1, Weidong Yang 1
1Department of Biology, Temple University

Recently we mapped the three-dimensional (3D) spatial locations of transport routes for various proteins translocating inside primary cilia in live cells. Here this paper details the experimental setup, the process of biological samples and the data analyses for the 3D super-resolution fluorescence imaging approach newly applied in live primary cilia.

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Education

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Menaka Ariyaratne 1, Lingxiao Ge 1, Paul F. Morris 1
1Department of Biological Sciences, Bowling Green State University

We describe a method for the characterization of proton-driven membrane transporters in membrane vesicle preparations produced by heterologous expression in E. coli and lysis of cells using a French press.

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