Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a HydrogelOlgu Enis Tok 1,2, Gamze Demirel 2,3, Yusuf Saatci 1, Zeynep Akbulut 3,5, Ozgecan Kayalar 6, Ranan Gulhan Aktas 2,3,4
1Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, 2Cellorama, 3Cancer and Stem Cell Research Center, Maltepe University, 4Department of Histology and Embryology, School of Medicine, Maltepe University, 5Department of Medical Biology, School of Medicine, Maltepe University, 6School of Medicine, Koc University
The present study describes methodologies for culturing, freezing, thawing, processing, staining, labeling, and examining entire spheroids and organoids under various microscopes, while they remain intact in a hydrogel within a multipurpose device.
