Universidad de Valladolid

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Fluorescence and Bioluminescence Imaging of Subcellular Ca²⁺ in Aged Hippocampal Neurons

María Calvo-Rodríguez¹, Carlos Villalobos¹, Lucía Nuñez²

¹Instituto de Biologìa y Genética Molecular, Consejo Superior de Investigaciones Cientìficas, ²Departamento de Bioquìmica y Biologìa Molecular y Fisiologìa, Universidad de Valladolid

Intracellular Ca²⁺ remodeling in aging may contribute to excitotoxicity and neuron damage, processes mediated by Ca²⁺ overload. We aimed at investigating Ca²⁺ remodeling in the aging brain using fluorescence and bioluminescence imaging of cytosolic and mitochondrial Ca²⁺ in long-term cultures of rat hippocampal neurons, a model of neuronal aging.

JoVE Core

Preparation of a Corannulene-functionalized Hexahelicene by Copper(I)-catalyzed Alkyne-azide Cycloaddition of Nonplanar Polyaromatic Units

Celedonio M. Álvarez¹, Héctor Barbero¹, Sergio Ferrero¹

¹GIR MIOMeT, IU CINQUIMA, Área de Química Inorgánica, Universidad de Valladolid

Here, we present a protocol to synthesize a complex organic compound comprised of three nonplanar polyaromatic units, assembled easily with reasonable yields.

Engineering

Effective Analysis of Human Exposure Conditions with Body-worn Dosimeters in the 2.4 GHz Band

Silvia de Miguel-Bilbao¹, Juan Blas², Victoria Ramos¹

¹Telemedicine and e-Health Research Unit, Instituto de Salud Carlos III, ²Signal Theory and Communications, and Telematic Engineering Department, Universidad de Valladolid

This study describes a protocol to measure exposure levels in the 2.4 GHz band, avoiding the uncertainties caused by the use of personal exposimeters as measuring devices. These alterations of the exposure levels should be taken into account, especially in compliance testing, where exposure limits are defined from non-perturbed data.