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1 ARTICLES PUBLISHED IN JoVE

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Biology

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
Danette L. Daniels 1, Jacqui Méndez 1, Hélène Benink 1, Andrew Niles 1, Nancy Murphy 1, Michael Ford 2, Richard Jones 2, Ravi Amunugama 2, David Allen 2, Marjeta Urh 1
1Promega Corporation, 2MS Bioworks LLC

HaloTag technology is a multifunctional technology which has shown significant success in isolation of both small and large protein complexes from mammalian cells.  Here we highlight the advantages of this technology compared to existing alternatives and demonstrate its utility to study numerous aspects of protein function inside eukaryotic cells.

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