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University of Science and Technology of China

10 ARTICLES PUBLISHED IN JoVE

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Biology

Using the optokinetic response to study visual function of zebrafish
Su-Qi Zou 1, Wu Yin 1, Ming-Jing Zhang 1, Chun-Rui Hu 1, Yu-Bin Huang 1, Bing Hu 1
1Laboratory of Neurodevelopment and Repair, Department of Neurobiology and Biophysics, School of Life, University of Science and Technology of China (USTC)

Optokinetic response has been widely used to assess the visual functions of larval zebrafish. Nevertheless, the standard protocol for larval fish is not yet readily applicable in adults1-5. Here, we introduce how to measure the OKR of adult zebrafish using a new protocol which is established in our lab.

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Medicine

Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function
Ronald X. Xu 1, Kun Huang 2, Ruogu Qin 1, Jiwei Huang 1, Jeff S. Xu 1, Liya Ding 2, Urmila S. Gnyawali 3, Gayle M. Gordillo 3, Surya C. Gnyawali 3,4, Chandan K. Sen 3
1Department of Biomedical Engineering, The Ohio State University, 2Department of Biomedical Informatics, The Ohio State University, 3Comprehensive Wound Center, The Ohio State University, 4Department of Surgery, The Ohio State University

A dual-mode imaging system was developed for non-contact assessment of cutaneous tissue oxygenation and vascular function.

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Neuroscience

Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes
Su-Qi Zou 1, Chen Tian 1, Su-Tie Du 1, Bing Hu 1
1Laboratory of Neurodevelopement and Repair, University of Science and Technology of China

We introduce an efficient method to retrograde label retinal ganglion cells (RGCs) in adult zebrafish.

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Engineering

Evaluating Plasmonic Transport in Current-carrying Silver Nanowires
Mingxia Song 1, Arnaud Stolz 1, Douguo Zhang 2, Juan Arocas 1, Laurent Markey 1, Gérard Colas des Francs 1, Erik Dujardin 3, Alexandre Bouhelier 1
1Laboratoire Interdisciplinaire Carnot de Bourgogne CNRS-UMR 6303, Université de Bourgogne, 2Department of Optics and Optical Engineering, University of Science and Technology of China, 3CEMES, CNRS-UPR 8011

Silver nanowires can simultaneously transport electrons and optical information in the form of surface plasmons. A procedure is described here to realize such a shared circuitry and the limitations at propagating both information carriers are evaluated.

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Immunology and Infection

An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood
Ke Yang *1,2,3, Jiandong Wu *3,4, Ling Zhu 1, Yong Liu 1, Michael Zhang 5, Francis Lin 3,4,6,7
1Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, 2University of Science and Technology of China, 3Department of Physics and Astronomy, University of Manitoba, 4Department of Biosystems Engineering, University of Manitoba, 5Seven Oaks General Hospital, 6Department of Immunology, University of Manitoba, 7Department of Biological Sciences, University of Manitoba

This article provides the detailed method of performing a rapid neutrophil chemotaxis assay by integrating the on-chip neutrophil isolation from whole blood and the chemotaxis test on a single microfluidic chip.

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Behavior

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila
Lifen Jiang *1,2, Yinpeng Zhan *2,3, Yan Zhu 2,3
1School of Life Science, University of Science and Technology of China, 2State key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, 3University of Chinese Academy of Sciences

Quantitative food-intake assays with dyed food provide a robust and high-throughput means to evaluate feeding motivation. Combining the food consumption assay with thermogenetic and optogenetic screens is a powerful approach to investigate the neural circuits underlying appetite in adult Drosophila melanogaster.

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Developmental Biology

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity
Mengrou Liu *1, Chunsen Zhu *1, Shun Bai *2, Xin Li 1, Kaiqiang Fu 1, Lan Ye 1, Ke Zheng 1
1State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 2Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China

Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements.

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Immunology and Infection

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice
Meng Wang *1, Yuanyuan Huang *1, Wangpeng Gu 2,3, Haikun Wang 1
1CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 2School of Life Science, University of Science and Technology of China, 3State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences

This paper describes protocols of evaluating Tfh and GC B response in mouse model of influenza virus infection.

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Engineering

Multimodal 3D Printing of Phantoms to Simulate Biological Tissue
Canzhen Ma *1,2, Shuwei Shen *1,2, Guangli Liu 1, Siyue Guo 1,2, Buyun Guo 1,2, Jialuo Li 1,2, Kuiming Huang 1,2, Yidan Zheng 1, Pengfei Shao 1,2, Erbao Dong 1,2, Jiaru Chu 1, Ronald X. Xu 1,2,3
1Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, 2Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education Institutes, University of Science and Technology of China, 3Department of Biomedical Engineering, The Ohio State University

Spin coating, polyjet printing, and fused deposition modeling are integrated to produce multilayered heterogeneous phantoms that simulate structural and functional properties of biological tissue.

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Medicine

Avoiding Ischemia Reperfusion Injury in Liver Transplantation
Yixi Zhang *1,2,3, Changjun Huang *1,2,3, Weiqiang Ju *1,2,3, Qiang Zhao 1,2,3, Maogen Chen 1,2,3, Linhe Wang 1,2,3, Dongping Wang 1,2,3, Linwei Wu 1,2,3, Zebin Zhu 4, Shanzhou Huang 5, Zhiheng Zhang 1,2,3, Caihui Zhu 1,2,3, Rongxing Xie 1,2,3, Yinghua Chen 1,2,3, Anbin Hu 1,2,3, Xiaofeng Zhu 1,2,3, Zhiyong Guo 1,2,3, Xiaoshun He 1,2,3
1Organ Transplant Center, The First Affiliated Hospital, Sun Yat-sen University, 2Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, 3Guangdong Provincial International Cooperation Base of Science and Technology, 4Organ Transplant Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, 5Department of General Surgery, Guangdong General Hospital, Guangdong Academy of Medical Sciences

Presented here is a protocol to provide a step-by-step ischemia-free liver transplantation protocol under ex situ normothermic machine perfusion (37 °C) of human livers from donors to recipients.

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