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Tokyo University of Science

9 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology
Xiaofeng Cui *1,2,3, Guifang Gao *2,4, Tomo Yonezawa 5,6, Guohao Dai 1
1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 2Stemorgan Inc., 3Institute of Advanced Study, Technical University of Munich, 4Institute of Virology, School of Medicine, Wuhan University, 5Department of Molecular and Experimental Medicine, The Scripps Research Institute, 6Research Institute for Biomedical Sciences, Tokyo University of Science

The methods described in this paper show how to convert a commercial inkjet printer into a bioprinter with simultaneous UV polymerization. The printer is capable of constructing 3D tissue structure with cells and biomaterials. The study demonstrated here constructed a 3D neocartilage.

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Bioengineering

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
Morito Sakuma 1, Yoshikazu Kumashiro 2, Masamichi Nakayama 2, Nobuyuki Tanaka 2, Yuji Haraguchi 2, Kazuo Umemura 1, Tatsuya Shimizu 2, Masayuki Yamato 2, Teruo Okano 2
1Department of Physics, Tokyo University of Science, 2Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University

Nanoscaled sea-island surfaces composed of thermoresponsive block copolymers were fabricated by the Langmuir-Schaefer method for controlling spontaneous cell adhesion and detachment. Both the preparation of the surface and the adhesion and detachment of cells on the surface were visualized.

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Chemistry

Preparation of Hollow Polystyrene Particles and Microcapsules by Radical Polymerization of Janus Droplets Consisting of Hydrocarbon and Fluorocarbon Oils
Yutaka Takahashi 1, Mari Kano 1, Natsu Yanagisawa 1, Yukishige Kondo 1
1Department of Industrial Chemistry, Faculty of Engineering, Tokyo University of Science

A protocol for the fabrication of hollow polymer particles and microcapsules by radical polymerization using emulsions consisting of styrene, perfluoro-n-octane, and aqueous SDS (sodium dodecylsulfate) solution is presented.

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Chemistry

Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides
Hidehisa Someya 1, Taiki Itoh 1, Mebae Kato 1, Shin Aoki 1,2
1Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2Imaging Frontier Center, Tokyo University of Science

Here, we present protocols for the synthesis of disaccharide nucleosides by the regioselective O-glycosylation of ribonucleosides via a temporary protection of their 2',3'-diol moieties utilizing a cyclic boronic ester. This method applies to several unprotected nucleosides such as adenosine, guanosine, cytidine, uridine, 5-methyluridine, and 5-fluorouridine to give corresponding disaccharide nucleosides.

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Engineering

Fabrication of an Optical Cell Dryer for the Spectroscopic Analysis Cells
Yuji Matsukawa 1, Shingo Shomura 1, Kazuo Umemura 1
1Department of Physics, Graduate School of Science, Tokyo University of Science

A protocol for fabricating a device for simultaneously drying multiple optical cells is presented.

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Engineering

A Photonic System for Generating Unconditional Polarization-Entangled Photons Based on Multiple Quantum Interference
Haruka Terashima 1, Yoshiro Sato 1, Satoshi Kobayashi 1, Takaho Tsubakiyama 1, Ryo Nozaki 1, Satoshi Kubo 1, Tomo Osada 1, Kaoru Sanaka 1
1Department of Physics, Tokyo University of Science

We describe an optical system for the generation of unconditional polarization-entangled photons based on multiple quantum interference effects with a detection scheme to estimate the experimental fidelity of generated entangled photons.

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Engineering

Fabrication of Microscope Stage for Vertical Observation with Temperature Control Function
Yuji Matsukawa 1, Yuki Ide 1, Kazuo Umemura 1
1Department of Physics, Graduate School of Science, Tokyo University of Science

Presented here is a protocol using a temperature-controlled microscope stage that allows a sample container to be mounted on a vertical microscope.

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Developmental Biology

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
Yu-Han Yeh *1,2, Mengwen Hu *1,2, Toshinori Nakagawa 3,4, Akihiko Sakashita 1,2, Shosei Yoshida 3,4, So Maezawa 5,6, Satoshi H. Namekawa 1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine, 3Division of Germ Cell Biology, National Institute for Basic Biology, National Institutes of Natural Sciences, 4Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (Sokendai), 5Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, 6Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science

Here we present a simple and efficient method to isolate live meiotic and post-meiotic germ cells from adult mouse testes. Using a low-cytotoxicity, violet-excited DNA binding dye and fluorescence-activated cell sorting, one can isolate highly enriched spermatogenic cell populations for many downstream applications.

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Engineering

Electroantennography-based Bio-hybrid Odor-detecting Drone using Silkmoth Antennae for Odor Source Localization
Daigo Terutsuki 1, Tomoya Uchida 2, Chihiro Fukui 3, Yuji Sukekawa 1, Yuki Okamoto 4, Ryohei Kanzaki 1
1Research Center for Advanced Science and Technology, The University of Tokyo, 2Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, 3Department of Applied Biological Science, Graduate School of Science and Technology, Tokyo University of Science, 4Sensing System Research Center, National Institute of Advanced Industrial Science and Technology

This study introduces experimental protocols for a bio-hybrid odor-detecting drone based on silkmoth antennae. The operation of an experimental electroantennogram device with silkmoth antennae is presented, in addition to the structure of a bio-hybrid drone designed for odor source localization using the spiral-surge algorithm.

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