Tokyo University of Science

9 ARTICLES PUBLISHED IN JoVE

Bioengineering

Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology

Xiaofeng Cui^*1,2,3, Guifang Gao^*2,4, Tomo Yonezawa^5,6, Guohao Dai¹

¹Department of Biomedical Engineering, Rensselaer Polytechnic Institute, ²Stemorgan Inc., ³Institute of Advanced Study, Technical University of Munich, ⁴Institute of Virology, School of Medicine, Wuhan University, ⁵Department of Molecular and Experimental Medicine, The Scripps Research Institute, ⁶Research Institute for Biomedical Sciences, Tokyo University of Science

The methods described in this paper show how to convert a commercial inkjet printer into a bioprinter with simultaneous UV polymerization. The printer is capable of constructing 3D tissue structure with cells and biomaterials. The study demonstrated here constructed a 3D neocartilage.

Bioengineering

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Morito Sakuma¹, Yoshikazu Kumashiro², Masamichi Nakayama², Nobuyuki Tanaka², Yuji Haraguchi², Kazuo Umemura¹, Tatsuya Shimizu², Masayuki Yamato², Teruo Okano²

¹Department of Physics, Tokyo University of Science, ²Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University

Nanoscaled sea-island surfaces composed of thermoresponsive block copolymers were fabricated by the Langmuir-Schaefer method for controlling spontaneous cell adhesion and detachment. Both the preparation of the surface and the adhesion and detachment of cells on the surface were visualized.

Chemistry

Preparation of Hollow Polystyrene Particles and Microcapsules by Radical Polymerization of Janus Droplets Consisting of Hydrocarbon and Fluorocarbon Oils

Yutaka Takahashi¹, Mari Kano¹, Natsu Yanagisawa¹, Yukishige Kondo¹

¹Department of Industrial Chemistry, Faculty of Engineering, Tokyo University of Science

A protocol for the fabrication of hollow polymer particles and microcapsules by radical polymerization using emulsions consisting of styrene, perfluoro-n-octane, and aqueous SDS (sodium dodecylsulfate) solution is presented.

Chemistry

Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides

Hidehisa Someya¹, Taiki Itoh¹, Mebae Kato¹, Shin Aoki^1,2

¹Faculty of Pharmaceutical Sciences, Tokyo University of Science, ²Imaging Frontier Center, Tokyo University of Science

Here, we present protocols for the synthesis of disaccharide nucleosides by the regioselective O-glycosylation of ribonucleosides via a temporary protection of their 2',3'-diol moieties utilizing a cyclic boronic ester. This method applies to several unprotected nucleosides such as adenosine, guanosine, cytidine, uridine, 5-methyluridine, and 5-fluorouridine to give corresponding disaccharide nucleosides.

Engineering

Fabrication of an Optical Cell Dryer for the Spectroscopic Analysis Cells

Yuji Matsukawa¹, Shingo Shomura¹, Kazuo Umemura¹

¹Department of Physics, Graduate School of Science, Tokyo University of Science

A protocol for fabricating a device for simultaneously drying multiple optical cells is presented.

Engineering

A Photonic System for Generating Unconditional Polarization-Entangled Photons Based on Multiple Quantum Interference

Haruka Terashima¹, Yoshiro Sato¹, Satoshi Kobayashi¹, Takaho Tsubakiyama¹, Ryo Nozaki¹, Satoshi Kubo¹, Tomo Osada¹, Kaoru Sanaka¹

¹Department of Physics, Tokyo University of Science

We describe an optical system for the generation of unconditional polarization-entangled photons based on multiple quantum interference effects with a detection scheme to estimate the experimental fidelity of generated entangled photons.

Engineering

Fabrication of Microscope Stage for Vertical Observation with Temperature Control Function

Yuji Matsukawa¹, Yuki Ide¹, Kazuo Umemura¹

¹Department of Physics, Graduate School of Science, Tokyo University of Science

Presented here is a protocol using a temperature-controlled microscope stage that allows a sample container to be mounted on a vertical microscope.

Developmental Biology

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Yu-Han Yeh^*1,2, Mengwen Hu^*1,2, Toshinori Nakagawa^3,4, Akihiko Sakashita^1,2, Shosei Yoshida^3,4, So Maezawa^5,6, Satoshi H. Namekawa^1,2

¹Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, ²Department of Pediatrics, University of Cincinnati College of Medicine, ³Division of Germ Cell Biology, National Institute for Basic Biology, National Institutes of Natural Sciences, ⁴Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (Sokendai), ⁵Department of Animal Science and Biotechnology, School of Veterinary Medicine, Azabu University, ⁶Faculty of Science and Technology, Department of Applied Biological Science, Tokyo University of Science

Here we present a simple and efficient method to isolate live meiotic and post-meiotic germ cells from adult mouse testes. Using a low-cytotoxicity, violet-excited DNA binding dye and fluorescence-activated cell sorting, one can isolate highly enriched spermatogenic cell populations for many downstream applications.

Engineering

Electroantennography-based Bio-hybrid Odor-detecting Drone using Silkmoth Antennae for Odor Source Localization

Daigo Terutsuki¹, Tomoya Uchida², Chihiro Fukui³, Yuji Sukekawa¹, Yuki Okamoto⁴, Ryohei Kanzaki¹

¹Research Center for Advanced Science and Technology, The University of Tokyo, ²Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, ³Department of Applied Biological Science, Graduate School of Science and Technology, Tokyo University of Science, ⁴Sensing System Research Center, National Institute of Advanced Industrial Science and Technology

This study introduces experimental protocols for a bio-hybrid odor-detecting drone based on silkmoth antennae. The operation of an experimental electroantennogram device with silkmoth antennae is presented, in addition to the structure of a bio-hybrid drone designed for odor source localization using the spiral-surge algorithm.