Kanazawa Medical University

5 ARTICLES PUBLISHED IN JoVE

Neuroscience

Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae

Maurizio Pellegrino¹, Takao Nakagawa¹, Leslie B. Vosshall¹

¹Laboratory of Neurogenetics and Behavior, Rockefeller University

Electrophysiological responses of olfactory sensory neurons to odorants can be measured in insects using single sensillum recordings. In this video article we will demonstrate how to perform single sensillum recordings in the antennae of the vinegar fly (Drosophila melanogaster) and the maxillary palps of the malaria mosquito (Anopheles gambiae).

Developmental Biology

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

Hidehito Saito^1,2, Kumiko Iwabuchi³, Noemi Fusaki^4,5, Fumito Ito^3,6

¹Department of Surgery, University of Michigan, ²Department of Biochemistry II, Kanazawa Medical University, ³Center for Immunotherapy, Roswell Park Cancer Institute, ⁴DNAVEC Corporation, ⁵Department of Ophthalmology, Keio University School of Medicine, ⁶Department of Surgical Oncology, Roswell Park Cancer Institute

The goal of this protocol is to show the protocol for reprogramming melanoma tumor-infiltrating lymphocytes into induced pluripotent stem cells.

Neuroscience

Optogenetics Identification of a Neuronal Type with a Glass Optrode in Awake Mice

Munenori Ono¹, Shinji Muramoto¹, Lanlan Ma¹, Nobuo Kato¹

¹Department of Physiology, Kanazawa Medical University

This work introduces a method to perform an optogenetic single-unit recording reliably from an awake mouse using a custom-made glass optrode.

Education

Investigation into Deep Breathing through Measurement of Ventilatory Parameters and Observation of Breathing Patterns

Masami Yokogawa¹, Tomoyo Kurebayashi², Kazuki Soma³, Hiroichi Miaki¹, Takao Nakagawa⁴

¹Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, ²Rehabilitation Section, Higashimatsuyama Municipal Hospital, ³Division of Health Sciences, Graduate School of Medical Sciences, Kanazawa University, ⁴Department of Physical and Rehabilitation Medicine, Kanazawa Medical University

Here, we present a protocol to assess two deep breathing patterns of natural and diaphragmatic breathing for their effectiveness and ease of execution. Fifteen participants were selected, utilizing an electrocardiograph and expired gas analyzer for measurement of the ventilatory parameters, together with visual assessment by video capture of thoracoabdominal movement.

Behavior

Modified Fear Conditioning for Inducing Flight Behaviors in Mice

Takafumi Furuyama^*1, Ryo Yamamoto^*1, Nobuo Kato¹, Munenori Ono¹

¹Department of Physiology, Kanazawa Medical University

To study flight behaviors in a fearful context, we introduce a modified fear conditioning protocol. This protocol ensures that mice consistently exhibit flight behaviors during cue presentation in the fear conditioning.