UNIVERSITY OF L'AQUILA

2 ARTICLES PUBLISHED IN JoVE

Medicine

Slow-release Drug Delivery through Elvax 40W to the Rat Retina: Implications for the Treatment of Chronic Conditions

Lavinia Fiorani^*1, Rita Maccarone^*1, Nilisha Fernando^2,3, Linda Colecchi¹, Silvia Bisti^1,2, Krisztina Valter^2,3,4

¹Biotechnology and Applied Clinical Sciences Department, University of L'Aquila, ²ARC Centre of Excellence in Vision Science, ³John Curtin School of Medical Research, Australian National University, ⁴ANU Medical School, Australian National University

This paper details how Elvax 40W can be used as a slow-release method for drug delivery to the adult rat retina. The protocol for preparing, loading, and delivering the drug-resin complex to the eye is described.

Genetics

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

Vincenzo De Iuliis¹, Sebastiano Ursi¹, Alfonso Pennelli², Marika Caruso², Sabrina Capodifoglio², Antonio Marino¹, Vincenzo Flati³, Gianfranco Vitullo¹, Elena Toniato², Iole Robuffo⁴, Stefano Martinotti^1,2

¹SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine, University of Chieti, ²Department of Medical, Oral and Biotechnological Sciences, University of Chieti, ³Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, ⁴CNR - Institute of Molecular Genetics, Section of Chieti

In the present study, we describe a methodology to analyze the C924T genotype. The protocol consists of three phases: DNA extraction, amplification by polymerase chain reaction (PCR), and analysis of the restriction fragment length polymorphism (RFLP) on agarose gel.