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University Hospital of Basel

5 ARTICLES PUBLISHED IN JoVE

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Medicine

Ischemic Tissue Injury in the Dorsal Skinfold Chamber of the Mouse: A Skin Flap Model to Investigate Acute Persistent Ischemia
Yves Harder 1, Daniel Schmauss 1, Reto Wettstein 2, José T. Egaña 1, Fabian Weiss 1, Andrea Weinzierl 1, Anna Schuldt 1, Hans-Günther Machens 1, Michael D. Menger 3, Farid Rezaeian 4
1Department of Plastic Surgery and Hand Surgery, Klinikum rechts der Isar, Technische Universität München, 2Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital of Basel, 3Institute for Clinical and Experimental Surgery, University of Saarland, 4Division of Plastic and Hand Surgery, University Hospital Zurich

The window of the murine dorsal skinfold chamber presented visualizes a zone of acute persistent ischemia of a musculocutaneous flap. Intravital epi-fluorescence microscopy permits for direct and repetitive assessment of the microvasculature and quantification of hemodynamics. Morphologic and hemodynamic results can further be correlated with histological and molecular analyses.

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Biology

Methylnitrosourea (MNU)-induced Retinal Degeneration and Regeneration in the Zebrafish: Histological and Functional Characteristics
Ellinor Maurer 1, Markus Tschopp 1,2, Christoph Tappeiner 1, Pauline Sallin 3, Anna Jazwinska 3, Volker Enzmann 1
1Department of Ophthalmology, Inselspital, University of Bern, 2Department of Ophthalmology, University Hospital of Basel, 3Department of Biology, University of Fribourg

Herein we demonstrate quantification of retinal de- and regeneration and its impact on visual function using N-methyl-N-nitrosourea in the adult zebrafish. Loss of visual acuity and decreased photoreceptor numbers were followed by proliferation in the inner nuclear layer. Complete morphological and functional regeneration occurred 30 days after the initial treatment.

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Immunology and Infection

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
Valerio Rossini 1, Katarina Radulovic 2, Christian U. Riedel 3, Jan Hendrik Niess 2
1APC Microbiome Institute, University College Cork, 2Division of Gastroenterology and Hepatology, University Hospital Basel, 3Institute of Microbiology and Biotechnology, University of Ulm

In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).

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Neuroscience

Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish
Federica M. Conedera 1,2,3, Petra Arendt 1, Carolyn Trepp 1,2,3, Markus Tschopp 1, Volker Enzmann 1,2
1Department of Ophthalmology, University Hospital of Bern, University of Bern, 2Department of Clinical Research, University of Bern, 3Graduate School for Cellular and Biomedical Sciences, University of Bern

The zebrafish is a popular animal model to study mechanisms of retinal degeneration/regeneration in vertebrates. This protocol describes a method to induce localized injury disrupting the outer retina with minimal damage to the inner retina. Subsequently, we monitor in vivo the retinal morphology and the Müller glia response throughout retinal regeneration.

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JoVE Journal

Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
Katarina Radulovic 1, Rachel Mak’Anyengo 1, Berna Kaya 1, Anna Steinert 1, Jan Hendrik Niess 1,2
1Department of Biomedicine, University of Basel, 2Division of Gastroenterology and Hepatology, University Hospital of Basel

Here a protocol to mimic the entrance of bacterial-derived compounds after intestinal barrier breach is presented. A low sublethal dose of lipopolysaccharide was injected systemically into mice, which were monitored for 24 h post-injection. The expression of pro-inflammatory cytokines was determined at several time points in spleen, liver, and colon.

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