Southern Medical University (China)

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice

Yi Yao^1,2, Yun Liu^1,3, Akira Takashima¹

¹Department of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, ²Department of Internal Medicine, Yale University School of Medicine, ³Department of Pathophysiology, Southern Medical University (China)

This current protocol employs fluorescent reporters, in vivo labeling, and intravital imaging techniques to enable monitoring of the dynamic process of neutrophil priming in living animals.

Biology

Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches

Jia-Hsin Huang¹, Yun Liu¹, Yu-Hsien Lin^1,2,3, Xavier Belles⁴, How-Jing Lee¹

¹Department of Entomology, National Taiwan University, ²Biology Centre, Institute of Entomology, Czech Academy of Sciences, ³Faculty of Science, University of South Bohemia, ⁴Institute of Evolutionary Biology, CSIC-UPF

This manuscript demonstrates the depletion of gene expression in the midgut of the German cockroach through oral ingestion of double-stranded RNA encapsulated in liposomes.

Medicine

Model of Ischemic Heart Disease and Video-Based Comparison of Cardiomyocyte Contraction Using hiPSC-Derived Cardiomyocytes

Yun Liu^*1, Yin Liang^*1, Mengxue Wang^*1, Chen Wang¹, Heng Wei², Keiji Naruse¹, Ken Takahashi¹

¹Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, ²Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

We present a model of ischemic heart disease using cardiomyocytes derived from human induced pluripotent stem cells, together with a method for quantitative evaluation of tissue damage caused by ischemia. This model can provide a useful platform for drug screening and further research on ischemic heart disease.