CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human CellsJing Ge *1, Somsak Prasongtanakij *2, David K. Wood 3, David M. Weingeist 1, Jessica Fessler 1, Panida Navasummrit 2, Mathuros Ruchirawat 2, Bevin P. Engelward 1
1Department of Biological Engineering, Massachusetts Institute of Technology, 2Environmental Toxicology, Chulabhorn Graduate Institute, 3Department of Biomedical Engineering, University of Minnesota
We describe here a platform that allows comet assay detection of DNA damage with unprecedented throughput. The device patterns mammalian cells into a microarray and enables parallel processing of 96 samples. The approach facilitates analysis of base level DNA damage, exposure-induced DNA damage and DNA repair kinetics.
