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University of North Dakota

7 ARTICLES PUBLISHED IN JoVE

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Biology

Bioenergetic Profile Experiment using C2C12 Myoblast Cells
David G. Nicholls 1, Victor M. Darley-Usmar 2, Min Wu 3, Per Bo Jensen 3, George W. Rogers 3, David A. Ferrick 3
1Buck Institute for Age Research, Novato, CA, 2Department of Pathology, Center for Free Radical Biology, University of Alabama at Birmingham - UAB, 3Seahorse Bioscience, North Billerica, MA

A description of a method for profiling mitochondrial function in cells is provided. The mitochondrial profile generated provides four parameters of mitochondrial function that can be measured in one experiment: basal respiration rate, ATP-linked respiration, proton leak, and reserve capacity.

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Genetics

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
Baoheng Gui *1,2,3, Yingxin Zhang *4, Bo Liang 5, Yvonne Ka Yin Kwok 4, Wai Ting Lui 4, Queenie Sum Yee Yeung 4, Lingyin Kong 6, Liming Xuan 6, Jacqueline Pui Wah Chung 4, Kwong Wai Choy 3,4
1Department of Genetics and Metabolism, Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, 2Birth Defects Prevention and Control Institute of Guangxi Zhuang Autonomous Region, 3Shenzhen Research Institute, The Chinese University of Hong Kong, 4Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, 5State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 6Basecare Medical Device Co., Ltd

Here, we introduce a semiconductor sequencing method for preimplantation genetic testing for aneuploidy (PGT-A) with the advantages of short turnaround time, low cost, and high throughput.

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Immunology and Infection

Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
Zhihan Wang *1,2, Qinqin Pu *1,2, Ping Lin *2,3, Changlong Li 1, Jianxin Jiang 3, Min Wu 2
1West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, 2Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, 3State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Army Medical University

This protocol describes techniques to measure infectious outcomes underlying secondary hospital-acquired infections in the immunosuppressive condition, first by establishing cecal ligation/puncture mice then challenging them with intranasal infection to create a clinically relevant model of immunosuppression sepsis.

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Immunology and Infection

Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin
Ramkumar Mathur 1,2,3, Mahabub Maraj Alam 4, Xiao-Feng Zhao 4, Yunfei Huang 4, Xinjun Zhu 1,2
1Department of Molecular and Cellular Physiology, Albany Medical College, 2The IBD Center, Division of Gastroenterology, Department of Medicine, Albany Medical College, 3Department of Geriatrics, School of Medicine and Health Science, University of North Dakota, 4Department of Neuroscience and Experimental Therapeutics, Albany Medical College

In this study, we describe a detailed procedure of TNBS-mediated intestinal fibrosis, which exhibits comparable pathophysiology to Crohn's fibrosis. We also discuss this approach in light of rapamycin facilitated inhibitory effects on intestinal fibrosis.

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Biochemistry

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
Yunrong Gao 1, Claire Ogilvie 1, Anirudh Raghavan 1, Chloe Von Hoffmann 1, Bo Liang 1
1Department of Biochemistry, Emory University School of Medicine

For in-depth mechanistic analysis of the respiratory syncytial virus (RSV) RNA synthesis, we report a protocol of utilizing the chaperone phosphoprotein (P) for coexpression of the RNA-free nucleoprotein (N0) for subsequent in vitro assembly of the virus-specific nucleocapsids (NCs).

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Neuroscience

Stereotaxic Viral Injection and Gradient-Index Lens Implantation for Deep Brain In Vivo Calcium Imaging
Rashmi Thapa 1, Bo Liang 2, Rongsong Liu 3, Yun Li 1
1Department of Zoology and Physiology, University of Wyoming, 2School of Electrical Engineering & Computer Science, University of North Dakota, 3Department of Mathematics and Statistics, University of Wyoming

Miniscope in vivo calcium imaging is a powerful technique to study neuronal dynamics and microcircuits in freely behaving mice. This protocol describes performing brain surgeries to achieve good in vivo calcium imaging using a miniscope.

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JoVE Core

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid
Ashton E. Enrriques 1, Sean Howard 2, Raju Timsina 3, Nawal K. Khadka 3, Amber N. Hoover 4, Allison E. Ray 5, Ling Ding 4, Chioma Onwumelu 6, Stephan Nordeng 6, Laxman Mainali 3,7, Gunes Uzer 2, Paul H. Davis 1,8
1Micron School of Materials Science & Engineering, Boise State University, 2Department of Mechanical & Biomedical Engineering, Boise State University, 3Department of Physics, Boise State University, 4Energy and Environmental Science and Technology, Idaho National Laboratory, 5Science and Technology, Idaho National Laboratory, 6Harold Hamm School of Geology & Geological Engineering, University of North Dakota, 7Biomolecular Sciences Graduate Program, Boise State University, 8Center for Advanced Energy Studies

Quantifying the contact area and force applied by an atomic force microscope (AFM) probe tip to a sample surface enables nanoscale mechanical property determination. Best practices to implement AFM cantilever-based nanoindentation in air or fluid on soft and hard samples to measure elastic modulus or other nanomechanical properties are discussed.

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