High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIREToshio Moriya 1, Michael Saur 1, Markus Stabrin 1, Felipe Merino 1, Horatiu Voicu 2, Zhong Huang 2, Pawel A. Penczek 2, Stefan Raunser 1, Christos Gatsogiannis 1
1Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, 2Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston
This paper presents a protocol for processing cryo-EM images using the software suite SPHIRE. The present protocol can be applied for nearly all single particle EM projects that target near-atomic resolution.