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The University of Texas Medical School at Houston

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
Toshio Moriya 1, Michael Saur 1, Markus Stabrin 1, Felipe Merino 1, Horatiu Voicu 2, Zhong Huang 2, Pawel A. Penczek 2, Stefan Raunser 1, Christos Gatsogiannis 1
1Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, 2Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston

This paper presents a protocol for processing cryo-EM images using the software suite SPHIRE. The present protocol can be applied for nearly all single particle EM projects that target near-atomic resolution.

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